1. Economic impact of Trichoderma
The industrial receiving of bio enzymes is ecologically and economically profitable. The increasing sales volume of Trichoderma fungi in the world market testifies to it. It increases with the annual dynamics in 10 %. Trichoderma harzianum, T reckon. hamatum and T. lignorum, and also their biotypes bring the greatest biological and commercial value.
The research of the nature of the growth and development of Trichoderma on some natural substrata for the development of the cultivation conditions of accumulative cultures showed that Trichoderma fungi do not demand any difficult and bulky modes of the cultivation, remote nutritious materials, ensuring high sterility at cultivation for its activity. Being an active antagonist of the majority of phito pathogenic fungi, Trichoderma can be widely applied for a biological control of fungal diseases of plants and has a big economic value. Moreover, at present the usage of Trichoderma preparations in the protected soil on a cucumber culture is economically profitable because there are always the hydrothermal conditions favorable for these fungi in the soil of hothouses.
“Trichoderma species play an important role in controlling fungal plant pathogens, especially soil borne fungal pathogens. The use of Trichoderma-based products is not only safe for the farmers, but also commercially profitable for consumers” (Ha: 2010).
2. Laboratory techniques and procedures used to sequence this fungus
The laboratory techniques used for the research of Trichoderma fungal genus are:
a total amount of protein is determined by the Kjeldahl method;
amino acid composition is determined on the automatic amino acid analyzer;
spectrophotometric method is used for the determination of tryptophane;
the technique of atomic absorption spectrometry is used for the measurement of the selenium concentration in the mycelium of fungi Trichoderma.
“These results demonstrated the utility of molecular techniques in indicating interrelations among species and, when combined with phenotypic characters, can lead to a sound taxonomy that is reflective of phylogenetic relationships” (Kinderman: 1998).
References
Boureghda, H., Z. Bouznad and C. Decock. (2008). “Cultural and Molecular Characterizations of Some Isolates of Trichoderma spp”. Arab J. Pl. Prot., 26: 75-80. Available at: HYPERLINK "http://www.asplantprotection.org/PDF/AJPP/26-1_2008/75-80.pdf" http://www.asplantprotection.org/PDF/AJPP/26-1_2008/75-80.pdf
Ha, T. (2010). “Using Trichoderma Species For Biological Control of Plant Pathogenes in Vietnam”. Available at: HYPERLINK "http://www.issaas.org/journal/v16/01/journal-issaas-v16n1-03-tranha.pdf " http://www.issaas.org/journal/v16/01/journal-issaas-v16n1-03-tranha.pdf
Kindermann, J., El-Ayouti, Yassin, Samuels, Gary J., Kubicek, Christian P. (1998). “Phylogeny of the Genus Trichoderma Based on Sequence Analysis of the Internal Transcribed Spacer Region 1 of the rDNA Cluster”. Fungal Genetics and Biology 24, 298-309. Available at: HYPERLINK "http://naldc.nal.usda.gov/download/35484/PDF" http://naldc.nal.usda.gov/download/35484/PDF
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